Allowed Sample types selected examples for CD measurements – EkkO – Spectroscopy – Application Note 30

Abstract

In this note, we demonstrate measurements for several CD standards covering the various classes of molecules that circular dichroism is used to study. Enantiomeric excess measurements for CSA and the unique in plate assays not available for traditional CD systems are also demonstrated.

Introduction

Circular dichroism (CD) is commonly used to assign the secondary structures of proteins and determining enantiomeric purities in asymmetric syntheses, both of which benefit from the ability to do the measurements in a high-throughput fashion. CD relies on the differential absorption between left and right circularly polarized light about a chiral center.

The primary advantage of the EKKO™ CD Microplate Reader is its speed resulting from the use of well plates allowing for the highest throughput possible. It accomplishes this by turning the light path from the horizontal used in traditional CD systems to vertical, allowing for a computer-controlled XY stage so that CD signals are read directly from a well plate instead of from a cuvette. This removes the time-consuming steps of 1) transferring the contents from each well of a well plate into a cuvette…

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