Rapid mixing stopped-flow small-angle X-ray scattering study of lipoplex formation at beamline ID02@ESRF

Borislav Angelov, Angelina Angelova, Markus Drechsler, Sylviane Lesieur

Journal of Surface Investigation. X-ray, Synchrotron and Neutron Techniques, January 2015, Volume 9, Issue 1, pp 105–110

Abstract

Rapid mixing stopped-flow experiments, coupled with synchrotron small-angle X-ray scattering (SAXS) on the subsecond timescale, produce a vast amount of experimental structural data. The purpose of this work is to present the key steps of the SAXS data treatment for the case of self-assembly and complexation of cationic lipid particles (vesicles or cubosomes) with plasmid DNA in the course of a rapid mixing stopped-flow process. This approach is significant for the structural analysis of rapidly forming soft-matter biomacro-molecular delivery systems.

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