RK-Spectro Technical Note 80: Single wavelength kinetic using MOS-500 in fluorescence mode

Introduction

In this application note, we explain how to perform single wavelength kinetics using MOS-500 in fluorescence mode, a stopped-flow module, and Bio-Kine32. The scenario presupposes that the A/D board has been correctly installed and Bio-kine32 correctly configured. In a 6M GndHCl solution hen egg lysozyme is unfolded, the refolding can be followed by a 1:9 dilution with water.