RK-Spectro Technical Note 81: Single wavelength kinetic using MOS-500 in absorbance mode

Introduction

In this Technical Note, we explain how to perform a single wavelength kinetics experiment using MOS500, a stopped-flow module, and Bio-Kine32. The scenario presupposes that the A/D board has been installed and Bio-kine32 correctly configured. The reduction of 2,6 dichlorophenolindophenol by L-sodium ascorbate is presented in this application note, the kinetics is followed by absorbance at 524 nm. If the concentration of DCIP is sufficiently smaller than sodium ascorbate then the reaction could be treated as a pseudo-first-order reaction.