RK-Spectro Technical Note 84: Single wavelength kinetic using MOS-500 in anisotropy mode

Introduction

The aim of this application note is to learn how to perform a single wavelength kinetics using MOS-500 in anisotropy mode, a stopped-flow module and Biokine32 (in this mode both fluorescence and anisotropy traces are displayed).
The scenario presupposes that the A/D board has been correctly installed and Bio-kine32 configured correctly. In a 6M GndHCl solution hen egg lysozyme is unfolded, the refolding can be followed by a 1:9 dilution with water.